Here is the conference alignment for the upcoming 2017 USL Season
| Western Conference | Eastern Conference |
| Colorado Springs Switchbacks FC | Bethlehem Steel FC |
| LA Galaxy II | Charleston Battery |
| OKC Energy FC | Charlotte Independence |
| Orange County Blues FC | FC Cincinnati |
| Phoenix Rising FC | Harrisburg City Islanders |
| Portland Timbers 2 | Louisville City FC |
| Real Monarchs SLC | New York Red Bulls II |
| Reno 1868 FC | Orlando City B |
| Rio Grande Valley FC | Ottawa Fury FC |
| Sacramento Republic FC | Pittsburgh Riverhounds |
| San Antonio FC | Richmond Kickers |
| Seattle Sounders FC 2 | Rochester Rhinos |
| Swope Park Rangers | Saint Louis FC |
| Tulsa Roughnecks FC | Tampa Bay Rowdies |
| Vancouver Whitecaps FC 2 | Toronto FC II |
The 2017 #USL Season will feature 495 games in 19 states as we continue to focus on growing regional rivalries. pic.twitter.com/tw97MlPlL0
— Jake Edwards (@EdwardsUSL) January 9, 2017
